图书简介
This 2005 text comprises 27 laboratory exercises for courses in developmental biology. These are experiments that provided insights into developmental questions, many of them presented by the scientists who carried out the original research. This book bridges experimental work and the laboratory classes taken at the undergraduate and post-graduate levels.
List of contributors; Preface Manuel Marí; Introduction Jennifer Knight; Part I. Graftings: 1. Two developmental gradients control head formation in hydra H. R. Bode; 2. Embryonic regulation and induction in sea urchin development C. A. Ettensohn; 3. The isthmic organizer and brain regionalization in chick embryos D. Echevaarría and S. Martínez; Part II. Specific Chemical Reagents: 4. Chemotaxis of aggregating Dictyostelium cells G. Gerisch and M. Ecke; 5. Inhibition of signal transduction pathways prevents head regeneration in hydra L. M. Salgado; 6. Retinoic acid during limb regeneration M. Maden; Part III. Beads Implantation: 7. Experimental techniques in avian embryos Y. Gañán, J. Rodríguez-León and D. Macías; 8. Induction of ectopic limb outgrowth in chick with FGF-8 A. Raya, C. Rodríguez Esteban and J. C. Izpisúa-Belmonte; Part IV. Nucleic Acid Injections: 9. RNAi techniques applied to freshwater planarians (Platyhelminthes) during regeneration D. Bueno, R. Romero and E. Saló; 10. Microinjection of Xenopus embryos R. J. Garriock and P. A. Krieg; Part V. Genetic Analysis: 11. Segmental specification in Drosophila melanogaster L. De Navas, M. Suzanne, D. Foronda and E. Sánchez-Herrero; 12. Genetic analysis of flower development in Arabidopsis thaliana: the ABC model of floral organ identity determination J. L. Riechmann; 13. Genetic analysis of vulva development in C. elegans S. Canevascini; Part VI. Clonal Analysis: 14. The role of the gene apterous in the development of the Drosophila wing F. J. Díaz-Benjumea; 15. Extramacrochaetae, an example of a gene required for control of limb size and cell differentiation during wing morphogenesis in Drosophila A. Baonza; 16. Hedgehog transduction pathway is involved in pattern formation in Drosophila melanogaster tergites M. Marí-Beffa; Part VII. In situ Hybridization: 17. Retinoic acid signalling controls anteroposterior patterning of the zebrafish hindbrain G. Begemann; 18. Left-right asymmetry in the mouse M. Blum, A. Schweickert and C. Karcher; Part VIII. Transgenic Organisms: 19. Bicoid and Dorsal: two transcriptions factor gradients which specify cell fates in the early Drosophila embryo S. Roth; 20. Significance of the temporal modulation of Hox gene expression on segment morphology J. Castelli-Gair Hombría; 21. The UAS/GAL4 system for tissue-specific analysis of EGFR gene function in Drosophila melanogaster J. B. Duffy and N. Perrimon; 22. Neurogenesis in Drosophila: a genetic approach C. Klämbt and H. Vaessin; 23. Role of the achaete-scute complex genes in the development of the adult peripheral nervous system of Drosophila melanogaster S. Sotillos and S. Campuzano; Part IX. Vertebrate Cloning: 24. The conservation of the genome and nuclear reprogramming in Xenopus J. B. Gurdon; Part X. Cell Culture: 25. In vitro culture and differentiation of mouse embryonic stem cells A. Rolletschek, C. Wiese and A. M. Wobus; Part XI. Evo-Devo Studies: 26. Microevolution between Drosophila species N. Skaer and P. Simpson; Part XII. Computational Modelling: 27. Theories as a tool to understand the complex network of molecular interactions M. Meinhardt; Appendices; Index.
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