图书简介
This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes. Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Cell Migration in Three Dimensions.- Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis.- Probing the ER-Focal Adhesion Link during Cell Migration.- Mapping the Localization of Proteins within Filopodia Using FiloMap.- Extended Methods for 2D Confinement.- Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63.- Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices.- Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila.- Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells.- Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure.- Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila.- A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland.- Analysis of Integrin-Dependent Melanoblast Migration during Development.- Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices.- Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion.- Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids.- Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices.- Analysis of Collective Migration Patterns within Tumors.- An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation.- Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior.- Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration.- Analysis of mRNA Subcellular Distribution in Collective Cell Migration.- A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta.- Analysis of Vascular Morphogenesis in Zebrafish.- Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐