Intestinal Differentiated Cells

图书简介

This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Intestinal Differentiated Cells: Methods and Protocols aims to be comprehensive guide for researchers.

Differentiated epithelial cells of the gut.- Identification of differentiated intestinal epithelial cells using immunostaining and fluorescence microscopy.- Novel approach to measure Trans Epithelial Electrical Resistance in intestinal cells.- In vivo model for isolating epithelial cells of the anorectal transition zone.- In vitro and in vivo assays for testing retinoids effect on intestinal progenitors’ lineage commitments.- TORNADO-seq: a protocol for high-throughput targeted RNA-seq based drug screening in organoids.- Epigenetic and transcriptional dynamics of Notch program in intestinal differentiation.- Defining anorectal transition zone heterogeneity using single-Cell RNA sequencing.- Directed differentiation of murine and human small intestinal organoids towards all mature lineages.- Modeling Notch activity and lineage decisions using intestinal organoids.- Generation of fetal Intestinal organoids and their maturation into adult intestinal cells.- Visualization of differentiated cells in 3D- and 2D-intestinal organoid cultures.- In-vitro culture and histological evaluation of 3D organotypic cultures.- Fluorescence intensity and fluorescence lifetime imaging (FLIM) microscopies of cell differentiation in the small intestinal organoids using cholera toxin.- In vitro morphogenesis and differentiation of human intestinal epithelium in a gut-on-a-chip.- Co-culturing human intestinal enteroid monolayers with innate immune cells.- Measuring cell differentiation in colorectal cancer.- Intestinal cell differentiation and phenotype in 2D and 3D cell culture models.- Confocal laser scanning imaging of cell junctions in human colon cancer cells.- Automated quantitative analysis of shape features in human epithelial monolayers and spheroids generated from colorectal cancer cells.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Intestinal Differentiated Cells（Methods in Molecular Biology） 肠分化细胞：方法与协议 细胞生物学

Intestinal Differentiated Cells（Methods in Molecular Biology）

肠分化细胞：方法与协议
细胞生物学