Crop Breeding:Genetic Improvement Methods

图书简介

This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Crop Breeding:Genetic Improvement Methodsaims to ensure successful results in the further study of this vital field.

Methods of Development of Biparental Mapping Populations in Horticultural Crops.-Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits.-Using Metabolomics to Assist Plant Breeding.-High Throughput DNA Isolation in Vegetable Crops for Genomics Applications.-High Resolution Melting Analysis as a Tool for Plant Species Authentication.-Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods.-Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome.-Association Mapping in Plants.-Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs).-Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis.-RNA Interference (RNAi) in Tomato Crop Reseserch.-ProtoplastBased Method for Genome Editing in TetraploidPotato.-The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria.-Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant.-Assembly of TALEN and mTALE-Act for Plant Genome Engineering.-Genome Editing to Achieve the Crop Ideotype in Tomato.-Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging.-Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.

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Crop Breeding:Genetic Improvement Methods（Methods in Molecular Biology） 作物育种：遗传改良方法 农艺学

Crop Breeding:Genetic Improvement Methods（Methods in Molecular Biology）

作物育种：遗传改良方法
农艺学